Category Archives: BIOTECHNOLOGY

BIOTECHNOLOGY AND BIOMEDICAL TUTORIAL-PCR, RFLP Analysis & Gene Therapy

The Polymerase Chain Reaction (PCR) Can Make Millions of Copies of DNA in a Short Time

  • The polymerase chain reaction (PCR) is a rapid way of amplifying (duplicating) specific DNA sequences
  • Method was devised by Kary Mullis of Cetus Corporation, Emeryville
    • He recieved a $20,000 bonus and later a Nobel Prize
    • Later the patent was sold to Hoffman-LaRoche for $300,000,000
  • DNA heated to high temperature is not destroyed; separates into single strands, but reforms helix when cooled
  • PCR Method:
    • DNA to be amplified is put into solution containing:
      • Short DNA “primers” which can bind to the 3′ ends of the DNA