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BIOTECHNOLOGY AND BIOMEDICAL TUTORIAL-PCR, RFLP Analysis & Gene Therapy

The Polymerase Chain Reaction (PCR) Can Make Millions of Copies of DNA in a Short Time

  • The polymerase chain reaction (PCR) is a rapid way of amplifying (duplicating) specific DNA sequences
  • Method was devised by Kary Mullis of Cetus Corporation, Emeryville
    • He recieved a $20,000 bonus and later a Nobel Prize
    • Later the patent was sold to Hoffman-LaRoche for $300,000,000
  • DNA heated to high temperature is not destroyed; separates into single strands, but reforms helix when cooled
  • PCR Method:
    • DNA to be amplified is put into solution containing:
      • Short DNA “primers” which can bind to the 3′ ends of the DNA

ANATOMY AND MECHANICS OF RESPIRATION-TUTORIAL

The Respiratory System is Designed to Bring in Oxygen and Remove Carbon Dioxide

  • A person with an average ventilation rate of 7.5 L/min will breathe in and out 10,800 liters of gas each day
  • From this gas the person will take in about 420 liters of oxygen (19 moles/day) and will give out about 340 liters of carbon dioxide (15 moles/day)
  • The ratio of CO2 expired/O2 inspired is called the respiratory quotient (RQ)
    • RQ = CO2 out/O2 in = 340/420 = 0.81
    • In cellular respiration of glucose CO2 out = O2 in; RQ = 1

SHORT NOTE ON PHOTOTHERAPY-BASIC CLINICAL SCIENCES

Light Therapy
Image by cabarney via Flickr

Phototherapy

Definition

Phototherapy, or light therapy, is the administration of doses of bright light in order to normalize the body‘s internal clock and/or relieve depression.

DESCRIPTION

Phototherapy is generally administered at home. The most commonly used phototherapy equipment is a portable lighting device known as a light box. The box may be mounted upright to a wall, or slanted downwards towards a table. The patient sits in front of the box for a prescribed period of time (anywhere from 15 minutes to several hours).

EYE AND ITS VISION-BASIC CLINICAL SCIENCES

Both Light and Sound Have Oscillating Waves

Velocity Travels Through Vacuum? Wavelength Frequency
Light 186,000 miles/sec
(300,000 km/sec)
in vacuum
Yes 400-700 nm 4 X 10^14
to 7 X 10 ^14
cycles/sec
Sound About 700 mph
(about 340 m/sec)
(1 mile per 5 seconds)
in air
No .02 to 20 meters 20-20,000 cycles/sec
  • Sound and light are waves in which the amplitude oscillates with time
    • For pure tones or colors the oscillation is a sine wave with a fixed wavelength and frequency
    • The wavelength is the distance between 2 peaks of the wave

TONOMETER-BASIC CLINICAL SCIENCES

Conventional surgery to treat glaucoma makes a...

Image via Wikipedia

WHAT IS TONOMETER?

THIS PARAGRAPH HAS BEEN WRITTEN IN REFERENCE TO MDU ROHTAK EXAM PATTERN

In order to ensure a person’s optic nerves are healthy, optometrists check the pressure placed on them by the fluid in the eyes. This pressure is called intraocular pressure and should measure between 10 mmHg and 21 mmHg. Measurements that are higher than normal can be a sign of early glaucoma or retinal detachment.

SHORT NOTES ON BASIC CLINICAL SCIENCES–EAR AND HEARING MECHANISM-

Sound is Produced by Vibrations in Matter (Air, Liquids, Solids)

  • Anything which causes matter to vibrate will produce sound
  • Vocal cords vibrate and push on the air flowing through the larynx, causing the air to vibrate
  • Sound cannot travel through a vacuum
  • Sound velocities:
    • Air: 344 meters/sec (770 miles/hr)
    • Water: 1500 meters/sec (3360 miles/hr)
    • Solids: about 5000 meters/sec (11,200 miles/hr)

Pitch is Determined by the Frequency of Vibration

  • We perceive fast vibrations as high pitches, slow vibrations as low pitches
  • When we are young we can hear vibrations from about 20 hertz to about 20,000 hertz