Workshop Dates
24th – 26th November 2011
ABOUT THE WORKSHOP
Dear Colleague:
On behalf of the Organisers, DNA Diagnostics, 2011, we take great pleasure in inviting you to the Hand’s on workshop on DNADiagnostics, 2011. The aim of this workshop is to update post-graduates, research scholars, pediatricians and gynecologist with the latest molecular techniques for the identification of genetic disorders.
This will help the participants to understand theoretical background and practical approaches with various applications. This is a golden opportunity to learn advanced techniques at affordable cost to apply and inculcate this knowledge in various applied areas of diagnostic research activities to be in forefront.
Organising Secretary
(Prof. Sarita Agarwal)
This is a preview of Hands on workshop on DNA Diagnostics,24th–26th November 2011,lucknow.
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Information is Stored in the Code Letters of DNA
- All hereditary information is stored in genes, which are parts of giant DNA molecules
- Genes code for the amino acids of proteins
- DNA is the archival copy of the code- kept in nucleus where it is protected & repaired
- DNA is organized with special proteins into chromosomes
- For protein synthesis a working copy of the code is made from RNA
- Overall scheme: DNA -> RNA -> protein
- Another version: “One gene, one enzyme”
The Code is Based Upon the Structure of DNA
Information is Stored in the Code Letters of DNA
- All hereditary information is stored in genes, which are parts of giant DNA molecules
- Genes code for the amino acids of proteins
- DNA is the archival copy of the code- kept in nucleus where it is protected & repaired
- DNA is organized with special proteins into chromosomes
- For protein synthesis a working copy of the code is made from RNA
- Overall scheme: DNA -> RNA -> protein
- Another version: “One gene, one enzyme”
The Polymerase Chain Reaction (PCR) Can Make Millions of Copies of DNA in a Short Time
- The polymerase chain reaction (PCR) is a rapid way of amplifying (duplicating) specific DNA sequences
- Method was devised by Kary Mullis of Cetus Corporation, Emeryville
- He recieved a $20,000 bonus and later a Nobel Prize
- Later the patent was sold to Hoffman-LaRoche for $300,000,000
- DNA heated to high temperature is not destroyed; separates into single strands, but reforms helix when cooled
- PCR Method:
- DNA to be amplified is put into solution containing:
- Short DNA “primers” which can bind to the 3′ ends of the DNA
This is a preview of BIOTECHNOLOGY AND BIOMEDICAL TUTORIAL-PCR, RFLP Analysis & Gene Therapy.
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